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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | kinesin family member 2C |
| Gene Symbol: | KIF2C |
| Synonyms: | MCAK; CT139; KNSL6 |
| Gene ID: | 11004 |
| UniProt ID: | Q99661 |
| Clone ID: | 24GB4930 |
| Immunogen: | A synthesized peptide derived from human MCAK |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a kinesin-like protein that functions as a microtubule-dependent molecular motor. The encoded protein can depolymerize microtubules at the plus end, thereby promoting mitotic chromosome segregation. Alternative splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-kinesin family member 2C antibody (Cat#63295). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-kinesin family member 2C antibody (Cat#63295, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-kinesin family member 2C antibody (Cat#63295). Kinesin family member 2C expression in wild type (WT) and kinesin family member 2C (KIF2C) shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-kinesin family member 2C antibody (Cat#63295, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using anti-NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of kinesin family member 2C expression in HepG2 cells using anti-kinesin family member 2C antibody (Cat#63295, 1:2,000). Green, isotype control; red, kinesin family member 2C. |
 | Immunocytochemical staining of HepG2 cells with anti-Kinesin family member 2C antibody (Cat#63295, 1:1,000). Nuclei were stained blue with DAPI; Kinesin family member 2C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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