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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | keratin 7 |
| Gene Symbol: | KRT7 |
| Synonyms: | K7; CK7; SCL; K2C7 |
| Gene ID: | 3855 |
| UniProt ID: | P08729 |
| Clone ID: | 24GB2830 |
| Immunogen: | Recombinant protein of human CytokeRatin 7. |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described.
Images
 | Western blotting analysis using anti-Keratin 7 antibody (Cat#62849). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Keratin 7 antibody (Cat#62849, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-keratin 7 antibody (Cat#62849). Keratin 7 expression in wild-type (WT) and keratin 7 (KRT7) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-keratin 7 antibody (Cat#62849, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Keratin 7 expression in HepG2 cells using anti-Keratin 7 antibody (Cat#62849, 1:2,000). Green, isotype control; red, Keratin 7. |
 | Immunocytochemical staining of HepG2 cells with anti-Keratin 7 antibody (Cat#62849, 1:1,000). Nuclei were stained blue with DAPI; Keratin 7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm. |
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