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| Reactivity: | Human |
| Applications: | WB |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | lactate dehydrogenase C |
| Gene Symbol: | LDHC |
| Synonyms: | CT32; LDH3; LDHX |
| Gene ID: | 3948 |
| UniProt ID: | P07864 |
| Clone ID: | 25GB4355 |
| Immunogen: | A synthesized peptide derived from human Lactate Dehydrogenase C |
| Dilution: | WB 1:1,000-1:5,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Lactate dehydrogenase C catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHC is testis-specific and belongs to the lactate dehydrogenase family. Two transcript variants have been detected which differ in the 5' untranslated region.
Images
 | Western blotting analysis using anti-lactate dehydrogenase C antibody (Cat#65579). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-lactate dehydrogenase C antibody (Cat#65579, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-lactate dehydrogenase C antibody (Cat#65579). Lactate dehydrogenase C expression in wild-type (WT) and lactate dehydrogenase C (LDHC) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-lactate dehydrogenase C antibody (Cat#65579, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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