KD-Validated LGALS1 Rabbit pAb (20 μl)

KD-Validated LGALS1 Rabbit pAb (20 μl)

Cat. #: 64961
Availability: In Stock
$149.00
-+
Reactivity:   Human, Mouse, Rat    
Applications:   WB    
Host Species:   Rabbit    
Isotype:   IgG    
Clonality:   Polyclonal antibody    
Gene Name:   galectin 1
Gene Symbol:   LGALS1
Synonyms:   GBP; GAL1
Gene ID:   3956
UniProt ID:   P09382
Immunogen:   A synthesized peptide derived from human Galectin 1
Dilution:   WB 1:500-1:2,500
Purification Method:   Affinity purified
Concentration:   Lot dependent
Buffer:   PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.




Background

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation.




Images

LGALS1 antibodyWestern blotting analysis using anti-galectin 1 antibody (Cat#64961). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-galectin 1 antibody (Cat#64961, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-galectin 1 antibody (Cat#64961). Galectin 1 expression in wild-type (WT) and galectin 1 (LGALS1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-galectin 1 antibody (Cat#64961, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).



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