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| Reactivity: | Human |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | lysophosphatidylcholine acyltransferase 1 |
| Gene Symbol: | LPCAT1 |
| Synonyms: | AYTL2; lpcat; AGPAT9; LPLAT8; PFAAP3; AGPAT10; LPCAT-1; lysoPAFAT |
| Gene ID: | 79888 |
| UniProt ID: | Q8NF37 |
| Clone ID: | 25GB180 |
| Immunogen: | Recombinant protein of human LPCAT1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a member of the 1-acyl-sn-glycerol-3-phosphate acyltransferase family of proteins. The encoded enzyme plays a role in phospholipid metabolism, specifically in the conversion of lysophosphatidylcholine to phosphatidylcholine in the presence of acyl-CoA. This process is important in the synthesis of lung surfactant and platelet-activating factor (PAF). Elevated expression of this gene may contribute to the progression of oral squamous cell, prostate, breast, and other human cancers.
Images
 | Western blotting analysis using anti-LPCAT1 antibody (Cat#65211). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-LPCAT1 antibody (Cat#65211, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-LPCAT1 antibody (Cat#65211). LPCAT1 expression in wild-type (WT) and LPCAT1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-LPCAT1 antibody (Cat#65211, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of LPCAT1 expression in HepG2 cells using anti-LPCAT1 antibody (Cat#65211, 1:2,000). Green, isotype control; red, LPCAT1. |
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