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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | macroH2A.1 histone |
| Gene Symbol: | MACROH2A1 |
| Synonyms: | H2A.y; H2A/y; H2AFY; mH2A1; H2AF12M; MACROH2A1.1; macroH2A1.2 |
| Gene ID: | 9555 |
| UniProt ID: | O75367 |
| Clone ID: | 25GB485 |
| Immunogen: | A synthesized peptide derived from human macroH2A.1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.
Images
 | Western blotting analysis using anti-MacroH2A.1 Histone antibody (Cat#61249). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MacroH2A.1 Histone antibody (Cat#61249, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-macroH2A.1 histone antibody (Cat#61249). MacroH2A.1 histone expression in wild type (WT) and macroH2A.1 histone (H2AFY) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-macroH2A.1 histone antibody (Cat#61249, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of MacroH2A.1 Histone expression in HAP-1 cells usiMacroH2A.1 Histone0 α antibody (Cat# 61249, 1:2,000). Green, isotype control; red, MacroH2A.1 Histone. |
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