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| Reactivity: | Human |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | mitotic arrest deficient 2 like 2 |
| Gene Symbol: | MAD2L2 |
| Synonyms: | REV7; FANCV; MAD2B; POLZ2 |
| Gene ID: | 10459 |
| UniProt ID: | Q9UI95 |
| Clone ID: | 24GB4950 |
| Immunogen: | A synthesized peptide derived from human Mad2L2 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. The encoded protein, which is similar to MAD2L1, is capable of interacting with ADAM9, ADAM15, REV1, and REV3 proteins.
Images
 | Western blotting analysis using anti-MAD2L2 antibody (Cat#63299). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MAD2L2 antibody (Cat#63299, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). MAD2L2, mitotic arrest deficient 2 like 2. |
 | Western blotting analysis using anti-MAD2L2 antibody (Cat#63299). MAD2L2 expression in wild-type (WT) and MAD2L2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-MAD2L2 antibody (Cat#63299, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of MAD2L2 expression in HAP-1 cells using anti-MAD2L2 antibody (Cat#63299, 1:2,000). Green, isotype control; red,MAD2L2 . |
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