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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | methyl-CpG binding domain protein 2 |
| Gene Symbol: | MBD2 |
| Synonyms: | DMTase; NY-CO-41 |
| Gene ID: | 8932 |
| UniProt ID: | Q9UBB5 |
| Clone ID: | 24GB460 |
| Immunogen: | A synthesized peptide derived from human MBD2 |
| Dilution: | WB 1:2,000-1:10,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. Two transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blotting analysis using anti-MBD2 antibody (Cat#62358). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MBD2 antibody (Cat#62358, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-MBD2 antibody (Cat#62358). MBD2 expression in wild type (WT) and MBD2 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MBD2 antibody (Cat#62358, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of MBD2 expression in HepG2 cells using MBD2 antibody (Cat#62358, 1:2,000). Green, isotype control; red, MBD2. |
 | Immunocytochemical staining of HepG2 cells with anti-MBD2 antibody (Cat#62358, 1:1,000). Nuclei were stained blue with DAPI; MBD2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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