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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | minichromosome maintenance complex component 5 |
| Gene Symbol: | MCM5 |
| Synonyms: | CDC46; MGORS8; P1-CDC46 |
| Gene ID: | 4174 |
| UniProt ID: | P33992 |
| Clone ID: | 24GB4965 |
| Immunogen: | A synthesized peptide derived from human MCM5 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.
Images
 | Western blotting analysis using anti-MCM5 antibody (Cat#63302). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MCM5 antibody (Cat#63302, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). MCM5, minichromosome maintenance complex component 5. |
 | Western blotting analysis using anti-MCM5 antibody (Cat#63302). MCM5 expression in wild-type (WT) and MCM5 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-MCM5 antibody (Cat#63302, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of MCM5 expression in HepG2 cells using anti-MCM5 antibody (Cat#63302, 1:2,000). Green, isotype control; red, MCM5. |
 | Immunocytochemical staining of HepG2 cells with anti-MCM5 antibody (Cat#63302, 1:1,000). Nuclei were stained blue with DAPI; MCM5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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