KD-Validated MFN1 Rabbit mAb (20 μl)

KD-Validated MFN1 Rabbit mAb (20 μl)

Cat. #: 63303
Availability: In Stock
$149.00
-+
Reactivity:   Human, Mouse, Rat    
Applications:   WB, FC    
Host Species:   Rabbit    
Isotype:   IgG    
Clonality:   Monoclonal antibody    
Gene Name:   mitofusin 1
Gene Symbol:   MFN1
Synonyms:   hfzo1; hfzo2
Gene ID:   55669
UniProt ID:   Q8IWA4
Clone ID:   24GB4970
Immunogen:   A synthesized peptide derived from Rat Mitofusin 1(UniProt Entry: Q8R4Z9)
Dilution:   WB 1:1,000-1:5,000; FC 1:2,000
Purification Method:   Affinity purified
Concentration:   Lot dependent
Buffer:   PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.




Background

The protein encoded by this gene is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting.




Images

MFN1 antibodyWestern blotting analysis using anti-mitofusin 1 antibody (Cat#63303). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-mitofusin 1 antibody (Cat#63303, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-mitofusin 1 antibody (Cat#63303). Mitofusin 1 expression in wild-type (WT) and mitofusin 1 (MFN1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-mitofusin 1 antibody (Cat#63303, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Mitofusin 1 expression in C2C12 cells using anti-Mitofusin 1 antibody (Cat#63303, 1:2,000). Green, isotype control; red, Mitofusin 1.



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