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| Reactivity: | Human |
| Applications: | WB, IC |
| Host Species: | Mouse |
| Isotype: | IgG1 kappa |
| Clonality: | Monoclonal antibody |
| Gene Name: | melanocyte inducing transcription factor |
| Gene Symbol: | MITF |
| Synonyms: | MI; WS2; CMM8; WS2A; COMMAD; MITF-A; bHLHe32 |
| Gene ID: | 4286 |
| UniProt ID: | O75030 |
| Clone ID: | 24GB2905 |
| Immunogen: | Recombinant protein of human MITF |
| Dilution: | WB 1:1,000-1:5,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. The encoded protein regulates melanocyte development and is responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome.
Images
 | Western blotting analysis using anti-Melanocyte inducing transcription factor antibody (Cat#62865). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Melanocyte inducing transcription factor antibody (Cat#62865, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-melanocyte inducing transcription factor antibody (Cat#62865). Melanocyte inducing transcription factor expression in wild-type (WT) and melanocyte inducing transcription factor (MITF) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-melanocyte inducing transcription factor antibody (Cat#62865, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Immunocytochemical staining of HepG2 cells with anti-Melanocyte inducing transcription factor antibody (Cat#62865, 1:1,000). Nuclei were stained blue with DAPI; Melanocyte inducing transcription factor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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