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| Reactivity: | Human, Mouse |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | mixed lineage kinase domain like pseudokinase |
| Gene Symbol: | MLKL |
| Synonyms: | hMLKL |
| Gene ID: | 197259 |
| UniProt ID: | Q8NB16 |
| Clone ID: | 24GB4620 |
| Immunogen: | A synthesized peptide derived from human MLKL |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene.
Images
 | Western blotting analysis using anti-MLKL antibody (Cat#63231). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MLKL antibody (Cat#63231, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). MLKL, mixed lineage kinase domain like pseudokinase. |
 | Western blotting analysis using anti-MLKL antibody (Cat#63231). MLKL expression in wild type (WT) and MLKL shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MLKL antibody (Cat#63231, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of MLKL expression in HepG2 cells using anti-MLKL antibody (Cat#63231, 1:2,000). Green, isotype control; red,MLKL. |
 | Immunocytochemical staining of HepG2 cells with anti-MLKL antibody (Cat#63231, 1:1,000). Nuclei were stained blue with DAPI; MLKL was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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