Reactivity: | Human, Mouse, Rat |
Applications: | WB, FC, IC |
Host Species: | Rabbit |
Isotype: | IgG |
Clonality: | Monoclonal antibody |
Gene Name: | moesin |
Gene Symbol: | MSN |
Synonyms: | HEL70; IMD50 |
Gene ID: | 4478 |
UniProt ID: | P26038 |
Clone ID: | 24GB265 |
Immunogen: | A synthesized peptide derived from human Moesin |
Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
Purification Method: | Affinity purified |
Concentration: | Lot dependent |
Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement.
Images
![]() | Western blotting analysis using anti-Moesin antibody (Cat#62313). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Moesin antibody (Cat#62313, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
![]() | Western blotting analysis using anti-Moesin antibody (Cat#62313). Moesin expression in wild type (WT) and Moesin shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Moesin antibody (Cat#62313, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
![]() | Flow cytometric analysis of Moesin expression in HepG2 cells using Moesin antibody (Cat#62313, 1:2,000). Green, isotype control; red, Moesin. |
![]() | Immunocytochemical staining of Hela cells with anti-Moesin antibody (Cat#62313, 1:1,000). Nuclei were stained blue with DAPI; Moesin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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