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| Reactivity: | Human, Mouse |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | methylenetetrahydrofolate reductase |
| Gene Symbol: | MTHFR |
| Synonyms: | Methylenetetrahydrofolate reductase |
| Gene ID: | 4524 |
| UniProt ID: | P42898 |
| Clone ID: | 25GB190 |
| Immunogen: | Recombinant protein of human MTHFR |
| Dilution: | WB 1:1,000-1:5,000; FC 1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine. Genetic variation in this gene influences susceptibility to occlusive vascular disease, neural tube defects, colon cancer and acute leukemia, and mutations in this gene are associated with methylenetetrahydrofolate reductase deficiency.
Images
 | Western blotting analysis using anti-MTHFR antibody (Cat#65213). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MTHFR antibody (Cat#65213, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-MTHFR antibody (Cat#65213). MTHFR expression in wild-type (WT) and MTHFR shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MTHFR antibody (Cat#65213, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of MTHFR expression in HeLa cells using anti-MTHFR antibody (Cat#65213, 1:2,000). Green, isotype control; red, MTHFR. |
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