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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | nicotinamide phosphoribosyltransferase |
| Gene Symbol: | NAMPT |
| Synonyms: | VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik |
| Gene ID: | 10135 |
| UniProt ID: | P43490 |
| Clone ID: | 25GB255 |
| Immunogen: | A synthesized peptide derived from human Visfatin |
| Dilution: | WB 1:1,000-1:5,000; FC 1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10.
Images
 | Western blotting analysis using anti-NAMPT antibody (Cat#65228). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NAMPT antibody (Cat#65228, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-NAMPT antibody (Cat#65228). NAMPT expression in wild-type (WT) and NAMPT shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NAMPT antibody (Cat#65228, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of NAMPT expression in HepG2 cells using anti-NAMPT antibody (Cat#65228, 1:2,000). Green, isotype control; red, NAMPT. |
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