Your shopping cart is empty!
| Reactivity: | Human |
| Applications: | WB, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | NADH:ubiquinone oxidoreductase subunit A13 |
| Gene Symbol: | NDUFA13 |
| Synonyms: | B16.6; CDA016; CGI-39; GRIM19; GRIM-19; MC1DN28 |
| Gene ID: | 51079 |
| UniProt ID: | Q9P0J0 |
| Clone ID: | 24GB600 |
| Immunogen: | A synthesized peptide derived from human GRIM19 |
| Dilution: | WB 1:1,000-1:5,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain. The protein is required for complex I assembly and electron transfer activity. The protein binds the signal transducers and activators of transcription 3 (STAT3) transcription factor, and can function as a tumor suppressor. The human protein purified from mitochondria migrates at approximately 16 kDa. Transcripts originating from an upstream promoter and capable of expressing a protein with a longer N-terminus have been found, but their biological validity has not been determined.
Images
 | Western blotting analysis using anti-NDUFA13 antibody (Cat#62387). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDUFA13 antibody (Cat#62387, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-NDUFA13 antibody (Cat#62387). NDUFA13 expression in wild type (WT) and NDUFA13 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NDUFA13 antibody (Cat#62387, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Immunocytochemical staining of HeLa cells with anti-NDUFA13 antibody (Cat#62387, 1:1,000). Nuclei were stained blue with DAPI; NDUFA13 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
You may also be interested in:
