Your shopping cart is empty!
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | NADH:ubiquinone oxidoreductase subunit B9 |
| Gene Symbol: | NDUFB9 |
| Synonyms: | B22; LYRM3; CI-B22; UQOR22; MC1DN24 |
| Gene ID: | 4715 |
| UniProt ID: | Q9Y6M9 |
| Clone ID: | 24GB90 |
| Immunogen: | A synthesized peptide derived from human NDUFB9 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a subunit of the mitochondrial oxidative phosphorylation complex I (nicotinamide adenine dinucleotide: ubiquinone oxidoreductase). Complex I is localized to the inner mitochondrial membrane and functions to dehydrogenate nicotinamide adenine dinucleotide and to shuttle electrons to coenzyme Q. Complex I deficiency is the most common defect found in oxidative phosphorylation disorders and results in a range of conditions, including lethal neonatal disease, hypertrophic cardiomyopathy, liver disease, and adult-onset neurodegenerative disorders. Pseudogenes of this gene are found on chromosomes five, seven and eight. Alternative splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-NDUFB9 antibody (Cat#62276). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDUFB9 antibody (Cat#62276, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-NDUFB9 antibody (Cat#62276). NDUFB9 expression in wild type (WT) and NDUFB9 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NDUFB9 antibody (Cat#62276, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of NDUFB9 expression in C2C12 cells using NDUFB9 antibody (Cat#62276, 1:2,000). Green, isotype control; red, NDUFB9. |
 | Immunocytochemical staining of C2C12 cells with anti-NDUFB9 antibody (Cat#62276, 1:1,000). Nuclei were stained blue with DAPI; NDUFB9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm. |
You may also be interested in:
