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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | NEDD4 like E3 ubiquitin protein ligase |
| Gene Symbol: | NEDD4L |
| Synonyms: | RSP5; PVNH7; NEDD4-2; NEDD4.2; hNEDD4-2 |
| Gene ID: | 23327 |
| UniProt ID: | Q96PU5 |
| Clone ID: | 25GB2910 |
| Immunogen: | A synthesized peptide derived from human NEDD4-2 |
| Dilution: | WB 1:4,000-1:20,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Images
 | Western blotting analysis using anti-NEDD4L antibody (Cat#61581). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NEDD4L antibody (Cat#61581, 1:20,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-NEDD4L antibody (Cat#61581). NEDD4L expression in wild-type (WT) and NEDD4L knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NEDD4L antibody (Cat#61581, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of NEDD4L expression in HepG2 cells using anti-NEDD4L antibody (Cat#61581, 1:2,000). Green, isotype control; red, NEDD4L. |
 | lmmunocytochemical staining of HepG2 cells with NEDD4L antibody (Cat#61581, 1:1,000). Nuclei were stained blue with DAPl; NEDD4L was stained magenta with Alexa Fluor® 647. lmages were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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