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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Mouse |
| Isotype: | IgG2a |
| Clonality: | Monoclonal antibody |
| Gene Name: | neural precursor cell expressed, developmentally down-regulated 9 |
| Gene Symbol: | NEDD9 |
| Synonyms: | CAS2; CASL; HEF1; CAS-L; CASS2 |
| Gene ID: | 4739 |
| UniProt ID: | Q14511 |
| Clone ID: | 25GB670 |
| Immunogen: | Recombinant protein of human NEDD9 |
| Dilution: | WB 1:400-1:2,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-NEDD9 antibody (Cat#65269). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NEDD9 antibody (Cat#65269, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-NEDD9 antibody (Cat#65269). NEDD9 expression in wild-type (WT) and NEDD9 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NEDD9 antibody (Cat#65269, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of NEDD9 expression in H9c2 cells using anti-NEDD9 antibody (Cat#65269, 1:2,000). Green, isotype control; red, NEDD9. |
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