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| Reactivity: | Human |
| Applications: | WB, FC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | neurofilament medium chain |
| Gene Symbol: | NEFM |
| Synonyms: | NFM; NEF3; NF-M |
| Gene ID: | 4741 |
| UniProt ID: | P07197 |
| Clone ID: | 24GB5825 |
| Immunogen: | Recombinant protein of human NEFM |
| Dilution: | WB 1:2,500-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Images
 | Western blotting analysis using anti-neurofilament medium chain antibody (Cat#63480). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-neurofilament medium chain antibody (Cat#63480, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-neurofilament medium chain antibody (Cat#63480). Neurofilament medium chain expression in wild type (WT) and neurofilament medium chain (NEFM) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-neurofilament medium chain antibody (Cat#63480, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Neurofilament medium chain expression in HeLa cells using anti-Neurofilament medium chain antibody (Cat#63480, 1:2,000). Green, isotype control; red, Neurofilament medium chain. |
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