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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | NIMA related kinase 6 |
| Gene Symbol: | NEK6 |
| Synonyms: | SID6-1512 |
| Gene ID: | 10783 |
| UniProt ID: | Q9HC98 |
| Clone ID: | 24GB755 |
| Immunogen: | A synthesized peptide derived from human NEK6 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a kinase required for progression through the metaphase portion of mitosis. Inhibition of the encoded protein can lead to apoptosis. This protein also can enhance tumorigenesis by suppressing tumor cell senescence. Several transcript variants encoding a few different isoforms have been found for this gene.
Images
 | Western blotting analysis using anti-NEK6 antibody (Cat#62421). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NEK6 antibody (Cat#62421, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-NEK6 antibody (Cat#62421). NEK6 expression in wild type (WT) and NEK6 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NEK6 antibody (Cat#62421, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of NEK6 expression in HT-1080 cells using NEK6 antibody (Cat#62421, 1:2,000). Green, isotype control; red, NEK6. |
 | Immunocytochemical staining of HT-1080 cells with anti-NEK6 antibody (Cat#62421, 1:1,000). Nuclei were stained blue with DAPI; NEK6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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