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| Reactivity: | Human, Rat |
| Applications: | WB |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | NIMA related kinase 9 |
| Gene Symbol: | NEK9 |
| Synonyms: | NC; APUG; NERCC; LCCS10; NERCC1 |
| Gene ID: | 91754 |
| UniProt ID: | Q8TD19 |
| Clone ID: | 24GB6355 |
| Immunogen: | Recombinant protein of human NEK9 |
| Dilution: | WB 1:2,500-1:5,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein is activated in mitosis and, in turn, activates other family members during mitosis. This protein also mediates cellular processes that are essential for interphase progression.
Images
 | Western blotting analysis using anti-NIMA related kinase 9 antibody (Cat#63526). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NIMA related kinase 9 antibody (Cat#63526, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-NIMA related kinase 9 antibody (Cat#63526). NIMA related kinase 9 expression in wild type (WT) and NIMA related kinase 9 (NEK9) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NIMA related kinase 9 antibody (Cat#63526, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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