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| Reactivity: | Human |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | NK2 homeobox 1 |
| Gene Symbol: | NKX2-1 |
| Synonyms: | BCH; BHC; NK-2; TEBP; TTF1; NKX2A; NMTC1; T/EBP; TITF1; TTF-1; NKX2.1 |
| Gene ID: | 7080 |
| UniProt ID: | P43699 |
| Clone ID: | 24GB5440 |
| Immunogen: | A synthesized peptide derived from human TTF1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The gene NKX2-1 encodes a protein initially identified as a thyroid-specific transcription factor. The encoded protein binds to the thyroglobulin promoter and regulates the expression of thyroid-specific genes but has also been shown to regulate the expression of genes involved in morphogenesis. Mutations and deletions in this gene are associated with benign hereditary chorea, choreoathetosis, congenital hypothyroidism, and neonatal respiratory distress, and may be associated with thyroid cancer. Multiple transcript variants encoding different isoforms have been found for this gene. This gene shares the symbol/alias 'TTF1' with another gene, transcription termination factor 1, which plays a role in ribosomal gene transcription.
Images
 | Western blotting analysis using anti-NK2 homeobox 1 antibody (Cat#63388). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NK2 homeobox 1 antibody (Cat#63388, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-NK2 homeobox 1 antibody (Cat#63388). NK2 homeobox 1 expression in wild type (WT) and NK2 homeobox 1 (NKX2-1) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NK2 homeobox 1 antibody (Cat#63388, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using anti-FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of NK2 homeobox 1 expression in HAP-1 cells using anti-NK2 homeobox 1 antibody (Cat#63388, 1:2,000). Green, isotype control; red, NK2 homeobox 1. |
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