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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | NAD(P) dependent 3-beta-hydroxysteroid dehydrogenase NSDHL |
| Gene Symbol: | NSDHL |
| Synonyms: | H105E3; XAP104; SDR31E1 |
| Gene ID: | 50814 |
| UniProt ID: | Q15738 |
| Clone ID: | 24GB485 |
| Immunogen: | A synthesized peptide derived from human NSDHL |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is localized in the endoplasmic reticulum and is involved in cholesterol biosynthesis. Mutations in this gene are associated with CHILD syndrome, which is a X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, and typically lethal in males. Alternatively spliced transcript variants with differing 5' UTR have been found for this gene.
Images
 | Western blotting analysis using anti-NSDHL antibody (Cat#62364). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NSDHL antibody (Cat#62364, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-NSDHL antibody (Cat#62364). NSDHL expression in wild type (WT) and NSDHL shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NSDHL antibody (Cat#62364, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of NSDHL expression in HeLa cells using NSDHL antibody (Cat#62364, 1:2,000). Green, isotype control; red, NSDHL. |
 | Immunocytochemical staining of HeLa cells with anti-NSDHL antibody (Cat#62364, 1:1,000). Nuclei were stained blue with DAPI; NSDHL was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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