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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | nuclear mitotic apparatus protein 1 |
| Gene Symbol: | NUMA1 |
| Synonyms: | NUMA; NMP-22 |
| Gene ID: | 4926 |
| UniProt ID: | Q14980 |
| Clone ID: | 24GB1290 |
| Immunogen: | A synthesized peptide derived from human NUMA1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a large protein that forms a structural component of the nuclear matrix. The encoded protein interacts with microtubules and plays a role in the formation and organization of the mitotic spindle during cell division. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 have been detected in patients with acute promyelocytic leukemia. Alternative splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-Nuclear mitotic apparatus protein 1 antibody (Cat#62534). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Nuclear mitotic apparatus protein 1 antibody (Cat#62534, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-Nuclear Mitotic Apparatus Protein 1 antibody (Cat#62534). Nuclear Mitotic Apparatus Protein 1 expression in wild type (WT) and Nuclear Mitotic Apparatus Protein 1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Nuclear Mitotic Apparatus Protein 1 antibody (Cat#62534, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Nuclear mitotic apparatus protein 1 expression in HepG2 cells using anti-Nuclear mitotic apparatus protein 1 antibody (Cat#62534, 1:2,000). Green, isotype control; red, Nuclear mitotic apparatus protein 1. |
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