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| Reactivity: | Human |
| Applications: | WB |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | OTU deubiquitinase, ubiquitin aldehyde binding 1 |
| Gene Symbol: | OTUB1 |
| Synonyms: | OTB1; OTU1; HSPC263 |
| Gene ID: | 55611 |
| UniProt ID: | Q96FW1 |
| Clone ID: | 24GB6500 |
| Immunogen: | Recombinant protein of human OTUB1 |
| Dilution: | WB 1:2,500-1:5,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-OTUB1 antibody (Cat#63556). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-OTUB1 antibody (Cat#63556, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-OTUB1 antibody (Cat#63556). OTUB1 expression in wild type (WT) and OTUB1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-OTUB1 antibody (Cat#63556, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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