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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | poly(A) binding protein interacting protein 1 |
| Gene Symbol: | PAIP1 |
| Synonyms: | Polyadenylate-binding protein-interacting protein 1; PABP-interacting protein 1; PAIP-1; Poly(A)-binding protein-interacting protein 1 |
| Gene ID: | 10605 |
| UniProt ID: | Q9H074 |
| Clone ID: | 25GB195 |
| Immunogen: | A synthesized peptide derived from human PAIP1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene interacts with poly(A)-binding protein and with the cap-binding complex eIF4A. It is involved in translational initiation and protein biosynthesis. Overexpression of this gene in COS7 cells stimulates translation. Alternative splicing occurs at this locus and three transcript variants encoding three distinct isoforms have been identified.
Images
 | Western blotting analysis using anti-PAIP1 antibody (Cat#65214). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PAIP1 antibody (Cat#65214, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-PAIP1 antibody (Cat#65214). PAIP1 expression in wild-type (WT) and PAIP1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PAIP1 antibody (Cat#65214, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of PAIP1 expression in HepG2 cells using anti-PAIP1 antibody (Cat#65214, 1:2,000). Green, isotype control; red, PAIP1. |
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