Your shopping cart is empty!
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | pyruvate dehydrogenase kinase 1 |
| Gene Symbol: | PDK1 |
| Synonyms: | PDHK1; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; Pyruvate dehydrogenase kinase isoform 1; PDH kinase 1 |
| Gene ID: | 5163 |
| UniProt ID: | Q15118 |
| Clone ID: | 24GB1190 |
| Immunogen: | A synthesized peptide derived from human PDK1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene.
Images
 | Western blotting analysis using anti-Pyruvate dehydrogenase kinase 1 antibody (Cat#62511). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Pyruvate dehydrogenase kinase 1 antibody (Cat#62511, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-Pyruvate dehydrogenase kinsae 1 antibody (Cat#62511). Pyruvate dehydrogenase kinsae 1 expression in wild type (WT) and Pyruvate dehydrogenase kinsae 1 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Pyruvate dehydrogenase kinsae 1 antibody (Cat#62511, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Pyruvate dehydrogenase kinase 1 expression in H9c2 cells using anti-Pyruvate dehydrogenase kinase 1 antibody (Cat#62511, 1:2,000). Green, isotype control; red, Pyruvate dehydrogenase kinase 1. |
You may also be interested in:
