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| Reactivity: | Human, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | Pancreatic and duodenal homeobox 1 |
| Gene Symbol: | PDX1 |
| Synonyms: | GSF; IPF1; IUF1; IDX-1; MODY4; PDX-1; STF-1; PAGEN1 |
| Gene ID: | 3651 |
| UniProt ID: | P52945 |
| Clone ID: | 24GB1380 |
| Immunogen: | A synthesized peptide derived from human PDX1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by the gene PDX1 is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. The encoded nuclear protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in this gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (IDDM), as well as maturity onset diabetes of the young type 4 (MODY4).
Images
 | Western blotting analysis using anti-PDX1 antibody (Cat#62554). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PDX1 antibody (Cat#62554, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-PDX1 antibody (Cat#62554). PDX1 expression in wild type (WT) and PDX1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . Hsp90 α serves as a loading control. The blot was incubated with anti-PDX1 antibody (Cat#62554, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of PDX1 expression in HeLa cells using anti-PDX1 antibody (Cat#62554, 1:2,000). Green, isotype control; red, PDX1. |
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