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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | phosphofructokinase, platelet |
| Gene Symbol: | PFKP |
| Synonyms: | PFKF; PFK-C; PFK-P; ATP-PFK |
| Gene ID: | 5214 |
| UniProt ID: | Q01813 |
| Clone ID: | 24GB6380 |
| Immunogen: | Recombinant protein of human PFKP |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a member of the phosphofructokinase A protein family. The encoded enzyme is the platelet-specific isoform of phosphofructokinase and plays a key role in glycolysis regulation. This gene may play a role in metabolic reprogramming in some cancers, including clear cell renal cell carcinomas, and cancer of the bladder, breast, and lung. Alternative splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-PFKP antibody (Cat#63531). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PFKP antibody (Cat#63531, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-PFKP antibody (Cat#63531). PFKP expression in wild type (WT) and PFKP knockdown (KD) HSHC cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PFKP antibody (Cat#63531, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of PFKP expression in HepG2 cells using anti-PFKP antibody (Cat#63531, 1:2,000). Green, isotype control; red, PFKP. |
 | Immunocytochemical staining of HepG2 cells with anti-PFKP antibody (Cat#63531, 1:1,000). Nuclei were stained blue with DAPI; PFKP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Medium. Scale bar, 20 μm. |
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