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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | phosphoglycerate mutase 1 |
| Gene Symbol: | PGAM1 |
| Synonyms: | PGAMA; PGAM-B; HEL-S-35 |
| Gene ID: | 5223 |
| UniProt ID: | P18669 |
| Clone ID: | 24GB1635 |
| Immunogen: | A synthesized peptide derived from human PGAM1 |
| Dilution: | WB 1:1,000-1:5,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a mutase that catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. Two transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blotting analysis using anti-Phosphoglycerate mutase 1 antibody (Cat#62609). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phosphoglycerate mutase 1 antibody (Cat#62609, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-Phosphogiycerate mutase 1 antibody (Cat#62609). Phosphogiycerate mutase 1 expression in wild type (WT) and Phosphogiycerate mutase 1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . β-Tubulin serves as a loading control. The blot was incubated with anti-Phosphogiycerate mutase 1 antibody (Cat#62609, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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