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| Reactivity: | Human, Mouse |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | phosphatidylinositol 3-kinase catalytic subunit type 3 |
| Gene Symbol: | PIK3C3 |
| Synonyms: | VPS34; Vps34; hVps34 |
| Gene ID: | 5289 |
| UniProt ID: | Q8NEB9 |
| Clone ID: | 24GB1150 |
| Immunogen: | A synthesized peptide derived from human PI 3 Kinase Class 3 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables 1-phosphatidylinositol-3-kinase activity. Involved in several processes, including early endosome to late endosome transport; macroautophagy; and phosphatidylinositol-3-phosphate biosynthetic process. Acts upstream of or within autophagy and positive regulation of protein lipidation. Located in autolysosome; late endosome; and midbody. Part of phosphatidylinositol 3-kinase complex, class III.
Images
 | Western blotting analysis using anti-PIK3C3 antibody (Cat#62502). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PIK3C3 antibody (Cat#62502, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-PIK3C3 antibody (Cat#62502). PIK3C3 expression in wild type (WT) and PIK3C3 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PIK3C3 antibody (Cat#62502, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of PIK3C3 expression in HeLa cells using PIK3C3 antibody (Cat#62502, 1:2,000). Green, isotype control; red, PIK3C3. |
 | Immunocytochemical staining of HeLa cells with anti-PIK3C3 antibody (Cat#62502, 1:1,000). Nuclei were stained blue with DAPI; PIK3C3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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