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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | phosphoinositide-3-kinase regulatory subunit 4 |
| Gene Symbol: | PIK3R4 |
| Synonyms: | p150; VPS15 |
| Gene ID: | 30849 |
| UniProt ID: | Q99570 |
| Clone ID: | 25GB325 |
| Immunogen: | A synthesized peptide derived from human PIK3R4 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Predicted to enable protein serine/threonine kinase activity. Involved in several processes, including early endosome to late endosome transport; phosphatidylinositol 3-kinase/protein kinase B signal transduction; and phosphatidylinositol-3-phosphate biosynthetic process. Located in late endosome and microtubule cytoskeleton. Part of phosphatidylinositol 3-kinase complex, class III.
Images
 | Western blotting analysis using anti-PIK3R4 antibody (Cat#65244). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PIK3R4 antibody (Cat#65244, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-PIK3R4 antibody (Cat#65244). PIK3R4 expression in wild-type (WT) and PIK3R4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PIK3R4 antibody (Cat#65244, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of PIK3R4 expression in HepG2 cells using anti-PIK3R4 antibody (Cat#65244, 1:2,000). Green, isotype control; red, PIK3R4. |
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