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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | piwi like RNA-mediated gene silencing 1 |
| Gene Symbol: | PIWIL1 |
| Synonyms: | HIWI; MIWI; PIWI; CT80.1 |
| Gene ID: | 9271 |
| UniProt ID: | Q96J94 |
| Clone ID: | 24GB1545 |
| Immunogen: | A synthesized peptide derived from human PIWIL1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. The encoded protein may play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blotting analysis using anti-PIWIL1 antibody (Cat#62591). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PIWIL1 antibody (Cat#62591, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-PIWIL1 antibody (Cat#62591). PIWIL1 expression in wild type (WT) and PIWIL1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . β-Tubulin serves as a loading control. The blot was incubated with anti-PIWIL1 antibody (Cat#62591, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of PIWIL1 expression in HT-1080 cells using anti-PIWIL1 antibody (Cat#62591, 1:2,000). Green, isotype control; red, PIWIL1. |
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