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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | protein kinase N1 |
| Gene Symbol: | PKN1 |
| Synonyms: | DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA |
| Gene ID: | 5585 |
| UniProt ID: | Q16512 |
| Clone ID: | 25GB1505 |
| Immunogen: | A synthesized peptide derived from human PKN1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Images
 | Western blotting analysis using anti-protein kinase N1 antibody (Cat#65428). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-protein kinase N1 antibody (Cat#65428, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-protein kinase N1 antibody (Cat#65428). Protein kinase N1A expression in wild-type (WT) and protein kinase N1 (PKN1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-protein kinase N1 antibody (Cat#65428, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of Protein kinase N1 expression in C2C12 cells using anti-Protein kinase N1 antibody (Cat#65428, 1:2,000). Green, isotype control; red, Protein kinase N1. |
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