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| Reactivity: | Human, Mouse |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | peptidylprolyl isomerase A |
| Gene Symbol: | PPIA |
| Synonyms: | CYPA; CYPH; HEL-S-69p |
| Gene ID: | 5478 |
| UniProt ID: | P62937 |
| Clone ID: | 24GB905 |
| Immunogen: | A synthesized peptide derived from human Cyclophilin A |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported.
Images
 | Western blotting analysis using anti-Peptidylprolyl isomerase A antibody (Cat#62452). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Peptidylprolyl isomerase A antibody (Cat#62452, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-peptidylprolyl isomerase A antibody (Cat#62452). Peptidylprolyl isomerase A expression in wild-type (WT) and peptidylprolyl isomerase A (PPIA) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-peptidylprolyl isomerase A antibody (Cat#62452, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Peptidylprolyl isomerase A expression in HepG2 cells using anti-Peptidylprolyl isomerase A antibody (Cat#62452, 1:2,000). Green, isotype control; red, Peptidylprolyl isomerase A. |
 | Immunocytochemical staining of HepG2 cells with anti-Peptidylprolyl isomerase A antibody (Cat#62452, 1:1,000). Nuclei were stained blue with DAPI; Peptidylprolyl isomerase A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm. |
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