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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | protein phosphatase 2 regulatory subunit B'epsilon |
| Gene Symbol: | PPP2R5E |
| Synonyms: | B56E; B56epsilon |
| Gene ID: | 5529 |
| UniProt ID: | Q16537 |
| Clone ID: | 24GB1215 |
| Immunogen: | A synthesized peptide derived from human PPP2R5E |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an epsilon isoform of the regulatory subunit B56 subfamily. Multiple transcript variants encoding several different isoforms have been found for this gene.
Images
 | Western blotting analysis using anti-PPP2R5E antibody (Cat#62517). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPP2R5E antibody (Cat#62517, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226) |
 | Western blotting analysis using anti-PPP2R5E antibody (Cat#62517). PPP2R5E expression in wild type (WT) and PPP2R5E shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . Hsp90 α serves as a loading control. The blot was incubated with anti-PPP2R5E antibody (Cat#62517, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of PPP2R5E expression in HepG2 cells using anti-PPP2R5E antibody (Cat#62517, 1:2,000). Green, isotype control; red, PPP2R5E. |
 | Immunocytochemical staining of HepG2 cells with anti-PPP2R5E antibody (Cat#62517, 1:1,000). Nuclei were stained blue with DAPI; PPP2R5E was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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