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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Host Species: | Mouse |
| Isotype: | IgG2b |
| Clonality: | Monoclonal antibody |
| Gene Name: | protein phosphatase 4 catalytic subunit |
| Gene Symbol: | PPP4C |
| Synonyms: | PP4; PPX; PP-X; PP4C; PPH3; PPP4 |
| Gene ID: | 5531 |
| UniProt ID: | P60510 |
| Clone ID: | 24GB13365 |
| Immunogen: | Recombinant protein of human PPP4C |
| Dilution: | WB 1:500-1:2,500 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables protein serine/threonine phosphatase activity. Involved in regulation of double-strand break repair via homologous recombination. Located in cytosol; nucleoplasm; and plasma membrane. Part of chromatin and protein phosphatase 4 complex.
Images
 | Western blotting analysis using anti-PPP4C antibody (Cat#64872). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPP4C antibody (Cat#64872, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-PPP4C antibody (Cat#64872). PPP4C expression in wild-type (WT) and PPP4C shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PPP4C antibody (Cat#64872, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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