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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | protein phosphatase 5 catalytic subunit |
| Gene Symbol: | PPP5C |
| Synonyms: | PP5; PPT; PPP5 |
| Gene ID: | 5536 |
| UniProt ID: | P53041 |
| Clone ID: | 24GB6665 |
| Immunogen: | Recombinant protein of human PPP5C |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a serine/threonine phosphatase which is a member of the protein phosphatase catalytic subunit family. Proteins in this family participate in pathways regulated by reversible phosphorylation at serine and threonine residues; many of these pathways are involved in the regulation of cell growth and differentiation. The product of this gene has been shown to participate in signaling pathways in response to hormones or cellular stress, and elevated levels of this protein may be associated with breast cancer development. Alternative splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-protein phosphatase 5 catalytic subunit antibody (Cat#63634). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-protein phosphatase 5 catalytic subunit antibody (Cat#63634, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-protein phosphatase 5 catalytic subunit antibody (Cat#63634). Protein phosphatase 5 catalytic subunit expression in wild type (WT) and protein phosphatase 5 catalytic subunit (PPP5C) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-protein phosphatase 5 catalytic subunit antibody (Cat#63634, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Protein phosphatase 5 catalytic subunit expression in HepG2 cells using anti-Protein phosphatase 5 catalytic subunit antibody (Cat#63634, 1:2,000). Green, isotype control; red, Protein phosphatase 5 catalytic subunit. |
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