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| Reactivity: | Human, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | peroxiredoxin 6 |
| Gene Symbol: | PRDX6 |
| Synonyms: | PRX; p29; AOP2; 1-Cys; NSGPx; aiPLA2; LPCAT-5; HEL-S-128m |
| Gene ID: | 9588 |
| UniProt ID: | P30041 |
| Clone ID: | 24GB910 |
| Immunogen: | A synthesized peptide derived from human PRDX6 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
Images
 | Western blotting analysis using anti-Peroxiredoxin 6 antibody (Cat#62453). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Peroxiredoxin 6 antibody (Cat#62453, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-Peroxiredoxin 6 antibody (Cat#62453). Peroxiredoxin 6 expression in wild type (WT) and Peroxiredoxin 6 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . β-Tubulin serves as a loading control. The blot was incubated with anti-Peroxiredoxin 6 antibody (Cat#62453, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Peroxiredoxin 6 expression in HepG2 cells using anti-Peroxiredoxin 6 antibody (Cat#62453, 1:2,000). Green, isotype control; red, Peroxiredoxin 6. |
 | Immunocytochemical staining of HepG2 cells with anti-Peroxiredoxin 6 antibody (Cat#62453, 1:1,000). Nuclei were stained blue with DAPI; Peroxiredoxin 6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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