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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | prolyl endopeptidase like |
| Gene Symbol: | PREPL |
| Synonyms: | CMS22 |
| Gene ID: | 9581 |
| UniProt ID: | Q4J6C6 |
| Clone ID: | 24GB995 |
| Immunogen: | A synthesized peptide derived from human PREPL |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene belongs to the prolyl oligopeptidase subfamily of serine peptidases. Mutations in this gene have been associated with hypotonia-cystinuria syndrome, also known as the 2p21 deletion syndrome. Several alternatively spliced transcript variants encoding either the same or different isoforms have been described for this gene.
Images
 | Western blotting analysis using anti-Prolyl endopeptidase like antibody (Cat#62470). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Prolyl endopeptidase like antibody (Cat#62470, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-Prolyl endopeptidase like antibody (Cat#62470). Prolyl endopeptidase like expression in wild type (WT) and Prolyl endopeptidase like shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . β-Tubulin serves as a loading control. The blot was incubated with anti-Prolyl endopeptidase like antibody (Cat#62470, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Prolyl endopeptidase like expression in H9c2 cells using anti-Prolyl endopeptidase like antibody (Cat#62470, 1:2,000). Green, isotype control; red, Prolyl endopeptidase like. |
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