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| Reactivity: | Human |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | protein kinase cAMP-dependent type II regulatory subunit alpha |
| Gene Symbol: | PRKAR2A |
| Synonyms: | PKR2; PRKAR2 |
| Gene ID: | 5576 |
| UniProt ID: | P13861 |
| Clone ID: | 24GB1265 |
| Immunogen: | A synthesized peptide derived from human PKA R2 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).
Images
 | Western blotting analysis using anti-PRKAR2A antibody (Cat#62527). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PRKAR2A antibody (Cat#62527, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-PRKAR2A antibody (Cat#62527). PRKAR2A expression in wild-type (WT) and PRKAR2A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . Hsp90 α serves as a loading control. The blot was incubated with anti-PRKAR2A antibody (Cat#62527, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of PRKAR2A expression in HAP-1 cells using anti-PRKAR2A antibody (Cat#62527, 1:2,000). Green, isotype control; red, PRKAR2A. |
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