Reactivity: | Human, Mouse, Rat |
Applications: | WB, FC |
Host Species: | Rabbit |
Isotype: | IgG |
Clonality: | Monoclonal antibody |
Gene Name: | protein kinase C delta |
Gene Symbol: | PRKCD |
Synonyms: | MAY1; PKCD; ALPS3; CVID9; nPKC-delta |
Gene ID: | 5580 |
UniProt ID: | Q05655 |
Clone ID: | 24GB4485 |
Immunogen: | Recombinant protein of Mouse PKC delta (UniProt Entry: P28867) |
Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
Purification Method: | Affinity purified |
Concentration: | Lot dependent |
Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrome.
Images
![]() | Western blotting analysis using anti-PKC delta antibody (Cat#63200). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PKC delta antibody (Cat#63200, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
![]() | Western blotting analysis using anti-PKC delta antibody (Cat#63200). PKC delta expression in wild-type (WT) and protein kinase C delta (PRKCD) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PKC delta antibody (Cat#63200, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
![]() | Flow cytometric analysis of PKC delta expression in HAP-1 cells using anti-PKC delta antibody (Cat#63200, 1:2,000). Green, isotype control; red,PKC delta. |
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