KD-Validated PTGES3 Rabbit mAb (20 μl)

KD-Validated PTGES3 Rabbit mAb (20 μl)

Cat. #: 62574
Availability: In Stock
$149.00
-+
Reactivity:   Human, Mouse, Rat    
Applications:   WB, FC    
Host Species:   Rabbit    
Isotype:   IgG    
Clonality:   Monoclonal antibody    
Gene Name:   prostaglandin E synthase 3
Gene Symbol:   PTGES3
Synonyms:   P23; TEBP; cPGES
Gene ID:   10728
UniProt ID:   Q15185
Clone ID:   24GB1470
Immunogen:   A synthesized peptide derived from human p23
Dilution:   WB 1:1,000-1:5,000; FC 1:2,000
Purification Method:   Affinity purified
Concentration:   Lot dependent
Buffer:   PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.




Background

This gene encodes an enzyme that converts prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). This protein functions as a co-chaperone with heat shock protein 90 (HSP90), localizing to response elements in DNA and disrupting transcriptional activation complexes. Alternative splicing results in multiple transcript variants. There are multiple pseudogenes of this gene on several different chromosomes.




Images

PTGES3 antibodyWestern blotting analysis using anti-Prostaglandin E synthase 3 antibody (Cat#62574). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Prostaglandin E synthase 3 antibody (Cat#62574, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-prostaglandin E synthase 3 antibody (Cat#62574). Prostaglandin E synthase 3 expression in wild-type (WT) and prostaglandin E synthase 3 (PTGES3) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-prostaglandin E synthase 3 antibody (Cat#62574, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Prostaglandin E synthase 3 expression in HepG2 cells using anti-Prostaglandin E synthase 3 antibody (Cat#62574, 1:2,000). Green, isotype control; red, Prostaglandin E synthase 3.



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