Your shopping cart is empty!
| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | protein tyrosine phosphatase non-receptor type 11 |
| Gene Symbol: | PTPN11 |
| Synonyms: | CFC; NS1; JMML; SHP2; BPTP3; PTP2C; METCDS; PTP-1D; SH-PTP2; SH-PTP3 |
| Gene ID: | 5781 |
| UniProt ID: | Q06124 |
| Clone ID: | 24GB5055 |
| Immunogen: | A synthesized peptide derived from human SHP2 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia.
Images
 | Western blotting analysis using anti-PTPN11 antibody (Cat#63323). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PTPN11 antibody (Cat#63323, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using Plus ECL Super Kit (Cat#636). PTPN11, protein tyrosine phosphatase non-receptor type 11. |
 | Western blotting analysis using anti-PTPN11 antibody (Cat#63323). PTPN11 expression in wild type (WT) and PTPN11 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PTPN11 antibody (Cat#63323, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of PTPN11 expression in HepG2 cells using anti-PTPN11 antibody (Cat#63323, 1:2,000). Green, isotype control; red,PTPN11. |
 | Immunocytochemical staining of HepG2 cells with anti-PTPN11 protein antibody (Cat#63323, 1:1,000). Nuclei were stained blue with DAPI; PTPN11 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
You may also be interested in:
