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| Reactivity: | Human |
| Applications: | WB, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | cyclin B1 |
| Gene Symbol: | CCNB1 |
| Synonyms: | CCNB |
| Gene ID: | 891 |
| UniProt ID: | P14635 |
| Clone ID: | 24GB4310 |
| Immunogen: | A synthesized peptide derived from human Phospho-Cyclin B1 (Ser126) |
| Dilution: | WB 1:1,000-1:5,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein CCNB1 encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle.
Images
 | Western blotting analysis using anti-phospho-cyclin B1 (Ser126) antibody (Cat#63161). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-cyclin B1 (Ser126) antibody (Cat#63161, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-phospho-cyclin B1 (Ser126) antibody (Cat#63161). Phospho-cyclin B1 (Ser126) expression in wild type (WT) and CCNB1 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-cyclin B1 (Ser126) antibody (Cat#63161, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Immunocytochemical staining of HepG2 cells with anti-phospho-cyclin B1 (Ser126) antibody (Cat#63161, 1:1,000). Nuclei were stained blue with DAPI; Phospho-cyclin B1 (Ser126) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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