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| Reactivity: | Human |
| Applications: | WB, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | kinesin family member 22 |
| Gene Symbol: | KIF22 |
| Synonyms: | KID; OBP; KNSL4; OBP-1; OBP-2; SEMDJL2; A-328A3.2 |
| Gene ID: | 3835 |
| UniProt ID: | Q14807 |
| Clone ID: | 25GB1650 |
| Immunogen: | A synthesized peptide derived from human KIF22 around the phosphorylation site of S427 |
| Dilution: | WB 1:1,000-1:5,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a member of the kinesin-like protein family. The family members are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. The C-terminal half of this protein has been shown to bind DNA. Studies with the Xenopus homolog suggests its essential role in metaphase chromosome alignment and maintenance. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blotting analysis using anti-phospho-KIF22 (S427) antibody (Cat#65473). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-KIF22 (S427) antibody (Cat#65473, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-phospho-KIF22 (S427) antibody (Cat#65473). Phospho-KIF22 (S427) expression in wild-type (WT) and KIF22 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-KIF22 (S427) antibody (Cat#65473, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Immunocytochemical staining of HepG2 cells with anti-Phospho-KIF22 (S427) antibody (Cat#65473, 1:1,000). Nuclei were stained blue with DAPI; Phospho-KIF22 (S427) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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