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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | presenilin 1 |
| Gene Symbol: | PSEN1 |
| Synonyms: | AD3; FAD; PS1; PS-1; S182; CMD1U; PSNL1; ACNINV3 |
| Gene ID: | 5663 |
| UniProt ID: | P49768 |
| Clone ID: | 24GB4510 |
| Immunogen: | A synthesized peptide derived from human Phospho-Presenilin 1 (Ser310) |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2) or in the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma-secretase activity or themselves are protease enzymes. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene, the full-length nature of only some have been determined.
Images
 | Western blotting analysis using anti-phospho-presenilin 1 (Ser310) antibody (Cat#63205). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-presenilin 1 (Ser310) antibody (Cat#63205, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-phospho-presenilin 1 (Ser310) antibody (Cat#63205). Phospho-presenilin 1 (Ser310) expression in wild-type (WT) and PSEN1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-phospho-presenilin 1 (Ser310) antibody (Cat#63205, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). PSEN1, presenilin 1. |
 | Flow cytometric analysis of Phospho-Presenilin 1 (Ser310) expression in HeLa cells using anti-Phospho-Presenilin 1 (Ser310) antibody (Cat#63205, 1:2,000). Green, isotype control; red, Phospho-Presenilin 1 (Ser310). |
 | Immunocytochemical staining of Hela cells with Phospho-Presenilin 1 (Ser310) antibody (Cat#63205, 1:1,000). Nuclei were stained blue with DAPI;Phospho-Presenilin 1 (Ser310) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium.Scale bar, 20 μm. |
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