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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | paxillin |
| Gene Symbol: | PXN |
| Synonyms: | Paxillin |
| Gene ID: | 5829 |
| UniProt ID: | P49023 |
| Clone ID: | 24GB900 |
| Immunogen: | A synthesized peptide derived from human Phospho-Paxillin (Y118) |
| Dilution: | WB 1:1,000-1:5,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445).
Images
 | Western blotting analysis using anti-Phospho-Paxillin (Y118) antibody (Cat#62450). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-Paxillin (Y118) antibody (Cat#62450, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-Phospho-Paxillin (Y118) antibody (Cat#62450). Phospho-Paxillin (Y118) expression in wild type (WT) and Phospho-Paxillin (Y118) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulinserves as a loading control. The blot was incubated with anti-Phospho-Paxillin (Y118) antibody (Cat#62450, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Immunocytochemical staining of Hela cells with anti-Phospho-Paxillin (Y118) antibody (Cat#62450, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Paxillin (Y118) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm. |
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