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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | vimentin |
| Gene Symbol: | VIM |
| Gene ID: | 7431 |
| UniProt ID: | P08670 |
| Clone ID: | 25GB3480 |
| Immunogen: | A synthesized peptide derived from human Phospho-Vimentin (Ser72) |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.
Images
 | Western blotting analysis using anti-phospho-vimentin (Ser72) antibody (Cat#61732). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-vimentin (Ser72) antibody (Cat#61732, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-phospho-vimentin (Ser72) antibody (Cat#61732). Phospho-vimentin (Ser72) expression in wild-type (WT) and VIM shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-vimentin (Ser72) antibody (Cat#61732, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of Phospho-Vimentin (Ser72) expression in HT-1080 cells using anti-Phospho-Vimentin (Ser72) antibody (Cat#61732, 1:2,000). Green, isotype control; red, Phospho-Vimentin (Ser72). |
 | Immunocytochemical staining of HT-1080 cells with anti-Phospho-Vimentin (Ser72) antibody (Cat#61732, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Vimentin (Ser72) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm. |
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